Study of the effect of gallic acid and cold plasma on the levels of inflammatory factors and antioxidants in the serum sample of subjects with type 2 diabetes mellitus


  • Danik Martirosyan PhD, Functional Food Institute, Dallas, TX, United States
  • Hamid Ghomi
  • Mohammad Reza Ashoori
  • Alireza Rezaeinezhad
  • Afsaneh Seyed Mikaeili
  • Fahimeh Jahanbakhshi
  • Hossein Mirmiranpour



Background: Uncontrolled type 2 diabetes mellitus can have devastating consequences. The role of functional foods in controlling and even preventing diabetes mellitus is prominent, and adjunct therapies can help control some of the consequences of diabetes. 

Objective: This study aimed to investigate whether gallic acid (as a functional food) and cold atmospheric plasma (as an adjunct therapy) influence the levels of some antioxidant enzymes, inflammatory factors, and the levels of an oxidizing agent and blood glucose.

Methods: In this study, 30 healthy individuals, as the control group, and 30 individuals with type 2 diabetes mellitus were selected. Samples of people with diabetes were examined before and after treatment with gallic acid and cold atmospheric plasma (cold argon plasma jet for 10 minutes). Levels of inflammatory factors (interleukin 2, 13 and NF-κB), antioxidants (glutathione reductase, paraoxonase, and lipoprotein lipase) as well as hydrogen peroxide and blood glucose, were assessed in untreated and treated diabetic groups and the control group according to kit instructions.

Results: A comparison of the results of the levels of inflammatory factors, antioxidants, blood glucose, and hydrogen peroxide showed a significant difference (P value < 0.05) between the diabetic and control groups. Treatment of diabetic subjects with plasma and gallic acid showed a significant increase (P value < 0.05) in glutathione reductase, paraoxonase, and NF-κB levels compared to the untreated diabetic group.

Conclusion: The results showed that concomitant use of gallic acid with plasma therapy, could be effective on NF-κB, glutathione reductase, and paraoxonase levels, yet the data suggest little or no effect. The results of the study showed that cold plasma treatment along with gallic acid supplementation can have a synergistic effect on the regulation of oxidative stress and inflammatory cytokine secretion in the patients with type 2 Diabetes mellitus.

Keywords: Diabetes mellitus, Cold plasma, Inflammatory factors, Gallic acid





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