Anti-inflammatory activity of Lactobacillus spp. and Rhodopseudomonas palustris probiotics
DOI:
https://doi.org/10.31989/bchd.v6i4.1067Abstract
Background: Probiotics have been used for many years to promote human health by mitigating inflammation. However, their mechanisms have not been fully elucidated. During inflammation, excessive and/or prolonged production of pro-inflammatory cytokines is related with various inflammatory diseases and cancer. Several probiotics have been reported as playing a role in suppressing the level of pro-inflammatory cytokines, as the human body attempts to recover itself. The aim of this study was to evaluate the anti-inflammatory activity of probiotics consisting of Lactobacillus spp. and Rhodopseudomonas palustris on macrophage RAW 264.7 cells.
Methods: The probiotics mixture was centrifuged to separate supernatant, i.e., the probiotics extract, from the cells. The probiotics extract was then evaluated for its effects on cell viability and for anti-inflammatory activity of LPS inflammation-induced RAW 264.7 cells.
Results: The results showed that the probiotics extract was able to decrease the level of IFN-γ, IL-1β, TNF-α, IL-8, TGF-β1 pro-inflammatory cytokines or mRNAs, and increase the level of IL-10 anti-inflammatory mRNA.
Conclusion: The probiotics extract was identified to have anti-inflammatory activity. Thus, the probiotics are promising to be used for treatment of chronic inflammatory conditions.
Keywords: multi-strain probiotics, anti-inflammatory cytokine/mRNA, RAW264.7
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