The potential of corn oil cake hydrolysate application as a substrate for Saccharomyces cerevisiae cultivation
DOI:
https://doi.org/10.31989/ffs.v6i1.1867Abstract
Background: The yeast Saccharomyces cerevisiae is a source of biologically active substances, which can be used in the development of functional food products. This drives the search for inexpensive, nutrient-rich substrates for the cultivation of this yeast. Such substrates can be based on agricultural by-products such as corn oil cake (COC). It is of particular interest due to its chemical composition, including high protein content (up to 25%), lipids (up to 16%), and carbohydrates (up to 42%).
Objective: This study aimed to evaluate the potential of using COC as a raw material for producing a substrate for the S. cerevisiae cultivation.
Methods: During the study, the chemical composition of COC was investigated by the AOAC methods. Enzymatic hydrolysis of COC was performed at a mash ratio of 1:10; at a temperature of 75°C for the first four hours and 60°C for the following four hours; and stirring at 500 rpm. After the completion of enzymatic hydrolysis, the hydrolysate was separated using a hydraulic press. Batch cultivation of S. cerevisiae L1 was performed at an initial concentration of dissolved solids of 6%, a temperature of 28 °C, for 24 hours.
Results: The COC had the following chemical composition: moisture – 4.74 ± 0.01 %; crude protein – 22.87 ± 0.58 % on a dry matter (DM) basis; lipids – 14.99 ± 0.47 % on a DM basis; crude fiber – 9.9 ± 1.4 % on a DM basis; and ash – 3.52 ± 0.51% on a DM basis. It has been shown that enzymatic hydrolysis of COC provides a dissolved solids (DS) yield of 46.24 ± 3.46% according to the mode proposed in this study. The use of COC hydrolysate as an additive to malt wort increased the efficiency of the cultivation process. Thus, at a COC hydrolysate concentration of 25% in the substrate composition, an increase in the growth coefficient and biomass yield was observed by 10.0 and 4.21%, respectively.
Conclusion: As a result of the work, it was demonstrated that the COC hydrolysate can be used as a substrate for the cultivation of S. cerevisiae. At the same time, when COC hydrolysate is used as an additive to malt wort, the efficiency of the S. cerevisiae cultivation process can be increased.
Keywords: corn oil cake, enzymatic hydrolysis, yeast cultivation, Saccharomyces cerevisiae
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Copyright (c) 2026 Vladimir Naumkin, Ekaterina Zasukhina, Tatiana Meledina, Dmitrii Manshin
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