Therapeutic potential of resveratrol, a polyphenol in the prevention of liver injury induced by diethylnitrosamine (DEN) through the regulation of inflammation and oxidative stress
DOI:
https://doi.org/10.31989/ffhd.v14i12.1502Abstract
Background: Resveratrol (RES) is an essential polyphenolic compound found in various foods, including vegetables, fruits, and chocolate. Its antioxidants and anti-inflammatory potential have attracted significant interest in the management of pathogenesis, including the treatment of hepatocellular carcinoma.
Objectives: This study was designed to measure RES hepatoprotective potential against Diethylnitrosamine (DEN)-induced liver injury.
Methods: The hepatoprotective potential of RES (20 mg/kg b.w) was evaluated by measuring the liver function enzymes, oxidative stress, and inflammation against DEN (200 mg/kg i.p)-induced liver injury. The liver tissue architecture and fibrosis were determined using hematoxylin and eosin, Masson trichrome, and Sirius Red staining. Moreover, the expression pattern of Cyclooxygense-2 (Cox-2) protein was evaluated by immunohistochemistry staining.
Results: Seven weeks of RES treatment after exposure to toxic DEN led to a significant reduction in liver function enzymes (AST, ALT, and ALP) and lipid peroxidation compared to rats treated by DEN only. Further, results showed that RES treatment reduced the pro-inflammatory cytokines (TNF-α, CRP, IL-1β, and IL-6) and increased the antioxidant enzymeFunctional Foods in Health and Disease 2024; 14(12): 898-920 FFHD Page 899 of 920 (Catalase, SOD, GST, and GPx) levels compared to rats treated by DEN only. The DEN-induced group of rats showed various liver tissue alterations, increased fibrosis, and high Cox-2 protein expression. However, the group treated with RES showed hepatoprotective potential with reduced liver tissue alterations and fibrosis. Moreover, RES treatment decreased the Cox-2 protein expression compared to rats treated by DEN only, suggesting its anti-inflammatory potential.
Conclusion: This study revealed that RES treatment reduced DEN-induced hepatic injury and liver cancer by protecting liver cells by reducing oxidative stress, inflammation, and liver function enzymes. Therefore, the results indicate that RES could be a possible therapeutic approach for liver diseases, including drug and alcohol-related problems.
Keywords: Resveratrol, Hepatoprotective, Oxidative stress, Inflammation and cancer, Alcohol-related problems, Fibrosis
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